a rapid and simple method of dna extraction from clinical specimens containing mycobacterium leprae for pcr assays
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abstract
we describe a rapid, simple, and reliable procedure for routine isolation of mycobacterium leprae dna from slit-skin swab specimens. this one-step dna extraction method is based on the utilization of chelexr 100, a chelating ion exchange resin. slit-skin swab specimens from seven untreated leprosy patients at baba baghi leprosy hospital in iran were processed by this procedure. the polymerase chain reaction (pcr) was performed with primers for a 530-base pair (bp) fragment of the gene encoding the 36 kda antigen of m. leprae. all the specimens were found to be pcr -positive, suggesting the efficiency of the present dna extraction method.
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Journal title:
medical journal of islamic republic of iranجلد ۱۰، شماره ۳، صفحات ۲۳۳-۲۳۵
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